Inactivation effect test of a new coronavirus
*TEST By: Biomedical Sciences Research Group (Narashino Research Institute)
Reported by Mikio Mizuno date; 2020 Oct. 26
Evaluation of Inactivation of new coronavirus in the environment by visible light-responsive photocatalysts
Test substance: Nanozone Solution (Visible Light Response) Rotating glass plate
Primer/Probe Set (2019-nCoV) (Takara Bio Inc. Product load XD0007)
Takara Bio Product XD0007
5x N_Sarbeco_Primer/Probe mix
500 n\
5x NlID_2019-nC0V_N_ Primer/Probe mix
500 pl
Test method
Sampling
Prepare the above Eiken Chemical Co., Ltd. wipe-off test. Wipe the floor of the hotel and office with 10cmX10cm in accordance with the manual. Move and save at 4 degrees C.
valuation of the effect of photocatalytic materials on new coronavirus (SARS-CoV-2) samples collected from the environment
Test objective: To evaluate the effect of photocatalytic materials on new coronavirus (SARS-CoV-2) samples collected from the environment.
Test substance (sample): photocatalyst sample with nanozone SOLUTION applied to borosilicate glass
Sample: nanozeon COAT (solution) coating on Rotating glass plate
Viruses used: collected from the environment
Test method JIS R 1702
(1) New coronavirus (SARS-CoV-2) environmental specimens were performed PCR test and Nested PCR test in accordance with the official Japanese law of the National Institute of Infectious Diseases.
(2) The glass plate 5cm x 5cm photocatalyst coated with nanozone SOLUTION was irradiated for 30 minutes with LED light source l, 000Lx.
The laboratory irradiated a 5cm x 5cm glass plate coated with a photocatalyst with nanozone solution for 30 minutes under an LED light source at l,000Lx
3 その後、PCRならびにNested PCRで電気泳動法にて解析した。
(3) After lighting, the laboratory analyzed by PCR and Nested PCR by electrophoresis.
The results are shown in the table below
Specimens to be used
New Coronavirus (SARS-CoV-2) Environmental Specimen
photocatalyst
Nanozone solution (COAT); coated glass plate
Comparison contrast
Un-coated glass plate
After 30min. irradiation
Below the detection limit
For the new coronavirus (SARS-CoV-2) sample collected from the environment, when processed with a photocatalytic carrier photocatalyst coated with nanozone SOLUTION, new coronavirus collected from the environment (SARS-CoV-2) analyzer begins to decompose instantaneously after irradiation under LED light source. In PCR and Nested PCR, it was below the detection limit after 30 minutes. Thus RNA of the new coronavirus collected from the environment (SARS-CoV-2) is degraded by visible light-responsive photocatalyst, it was observed as antiviral activity.